Amiloride,5-(N-Ethyl-N-isopropyl)-[5-(N-Ethyl-N-isopropyl)amiloride
EIPA
CAS: 1154-25-2
Molecular Formula: C11H18ClN7O
Amiloride,5-(N-Ethyl-N-isopropyl)-[5-(N-Ethyl-N-isopropyl)amiloride - Names and Identifiers
Amiloride,5-(N-Ethyl-N-isopropyl)-[5-(N-Ethyl-N-isopropyl)amiloride - Physico-chemical Properties
| Molecular Formula | C11H18ClN7O |
| Molar Mass | 299.76 |
| Density | 1.49±0.1 g/cm3(Predicted) |
| Melting Point | 202-205 °C(lit.) |
| Solubility | Acetonitrile (Slightly, Heated), DMSO (Slightly), Methanol (Slightly) |
| Appearance | Off-white to yellow solid. |
| Color | Pale Yellow to Green |
| pKa | 8.67±0.46(Predicted) |
| Storage Condition | Keep in dark place,Sealed in dry,2-8°C |
| Stability | Unstable in methanol |
| Physical and Chemical Properties | Bioactive EIPA (L593754) is a TRPP3 channel inhibitor with an IC50 value of 10.5 μM. EIPA also inhibits Na +/H + exchange (NHE) and macrophages. |
Amiloride,5-(N-Ethyl-N-isopropyl)-[5-(N-Ethyl-N-isopropyl)amiloride - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S37/39 - Wear suitable gloves and eye/face protection |
| UN IDs | 2811 |
| WGK Germany | 3 |
| Hazard Class | 6.1(b) |
| Packing Group | III |
Amiloride,5-(N-Ethyl-N-isopropyl)-[5-(N-Ethyl-N-isopropyl)amiloride - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 3.336 ml | 16.68 ml | 33.36 ml |
| 5 mM | 0.667 ml | 3.336 ml | 6.672 ml |
| 10 mM | 0.334 ml | 1.668 ml | 3.336 ml |
| 5 mM | 0.067 ml | 0.334 ml | 0.667 ml |
Last Update：2024-01-02 23:10:35
Amiloride,5-(N-Ethyl-N-isopropyl)-[5-(N-Ethyl-N-isopropyl)amiloride - Introduction
Nature:
1. It is a white to light yellow solid that dissolves in water and organic solvents.
2. It is a Na+/H+exchanger inhibitor that can be used to study the mechanism of sodium and hydrogen ion exchange within cells, as well as to regulate intracellular pH values.
Usage:
1. Used to study the mechanism of sodium and hydrogen ion exchange within cells.
2. Regulate intracellular pH levels.
Method:
The preparation method of 5- (N-ethyl-N-isopropyl) amiloride (EIPA) is relatively complex and is generally obtained through chemical synthesis.
Security information:
1. Rinse promptly when in contact with skin and eyes.
2. Avoid inhalation or ingestion, and avoid contact with fire sources.
3. When operating in ventilated conditions, wear appropriate protective equipment.
4. Try to avoid prolonged contact.
5. Pay attention to environmental protection when handling waste and comply with relevant regulations.
Last Update：2024-05-09 12:10:52
Amiloride,5-(N-Ethyl-N-isopropyl)-[5-(N-Ethyl-N-isopropyl)amiloride - Uses and synthesis methods
Target
IC50: 10.5 μM (TRPP3 channel)
NHE
Macropinocytosis
in vitro studies
In the presence of 100 μM EIPA, 10 μM benzamil, and 10 μM phenamil, 45 Ca 2+ uptake decreases from 79±9 to 46±4 (58% remaining), 27±4 (34%), 29±5 (37%), and 38±4 (48%) pmol/oocyte/30 min (n=6, P=0.008), respectively. It is found that EIPA, benzamil, and phenamil rapidly and reversibly block Ca 2+ -activated TRPP3 channel activation at -50 mV, with IC 50 s of 143±8 (n=36), 10.5±2.2 (n=28), 1.1±0.3 (n=30), and 0.14±0.04 μM (n=25), respectively. The number of autophagic vacuoles increases dramatically in the HAE and HPE groups after EIPA treatment compare with the HAN and HPN groups. EIPA regulates the initiation and maturation of the autophagy associated with amino acids in IEC-18 cells. In addition, the uptake of cinnamoylphenazine (CA-PZ) and neutral red (NR) is inhibited by EIPA.
Last Update：2024-05-09 12:11:22
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CAS: 1154-25-2
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
CAS: 1154-25-2
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Wechat: 18301782025
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